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31.
A new pressure-perfusion technique was used to measure hydraulic and osmotic properties of the outer part of roots (OPR) of 30-day-old rice plants (lowland cultivar: IR64, and upland cultivar: Azucena). The OPR comprised rhizodermis, exodermis, sclerenchyma and one cortical cell layer. The technique involved perfusion of aerenchyma of segments from two different root zones (20-50 mm and 50-100 mm from the tip) at precise rates using aerated nutrient solution. The hydraulic conductivity of the OPR (Lp(OPR)=1.2x10(-6) m s(-1) MPa(-1)) was larger by a factor of 30 than the overall hydraulic conductivity (Lp(r)=4x10(-8) m s(-1) MPa(-1)) as measured by pressure chamber and root pressure probe. Low reflection coefficients were obtained for mannitol and NaCl for the OPR (sigma(sOPR)=0.14 and 0.09, respectively). The diffusional water permeability ( P(dOPR)) estimated from isobaric flow of heavy water was smaller by three orders of magnitude than the hydraulic conductivity (Lp(OPR)/ P(fOPR)). Although detailed root anatomy showed well-defined Casparian bands and suberin lamellae in the exodermis, the findings strongly indicate a predominantly apoplastic water flow in the OPR. The Lp(OPR) of heat-killed root segments increased by a factor of only 2, which is in line with the conclusion of a dominating apoplastic water flow. The hydraulic resistance of the OPR was not limiting the passage of water across the root cylinder. Estimations of the hydraulic properties of aerenchyma suggested that the endodermis was rate-limiting the water flow, although the aerenchyma may contribute to the overall resistance. The resistance of the aerenchyma was relatively low, because mono-layered cortical septa crossing the aerenchyma ('spokes') short-circuited the air space between the stele and the OPR. Spokes form hydraulic bridges that act like wicks. Low diffusional water permeabilities of the OPR suggest that radial oxygen losses from aerenchyma to medium are also low. It is concluded that in rice roots, water uptake and oxygen retention are optimized in such a way that hydraulic water flow can be kept high in the presence of a low efflux of oxygen which is diffusional in nature. 相似文献
32.
We investigated the role of xylem cavitation, plant hydraulic conductance, and root pressure in the response of rice (Oryza sativa) gas exchange to water stress. In the field (Philippines), the percentage loss of xylem conductivity (PLC) from cavitation exceeded 60% in leaves even in watered controls. The PLC versus leaf water potential relationship indicated diurnal refilling of cavitated xylem. The leaf water potential causing 50 PLC (P(50)) was -1.6 MPa and did not differ between upland versus lowland rice varieties. Greenhouse-grown varieties (Utah) were more resistant to cavitation with a 50 PLC of -1.9 MPa but also showed no difference between varieties. Six-day droughts caused concomitant reductions in leaf-specific photosynthetic rate, leaf diffusive conductance, and soil-leaf hydraulic conductance that were associated with cavitation-inducing water potentials and the disappearance of nightly root pressure. The return of root pressure after drought was associated with the complete recovery of leaf diffusive conductance, leaf-specific photosynthetic rate, and soil-leaf hydraulic conductance. Root pressure after the 6-d drought (61.2 +/- 8.8 kPa) was stimulated 7-fold compared with well-watered plants before drought (8.5 +/- 3.8 kPa). The results indicate: (a) that xylem cavitation plays a major role in the reduction of plant hydraulic conductance during drought, and (b) that rice can readily reverse cavitation, possibly aided by nocturnal root pressure. 相似文献
33.
34.
Activation by Ca2+, Mg2+, Zn2+, or Mn2+ of adenosine triphosphatases in a microsomal fraction from wheat roots depends upon the growth temperature when the plants are grown under low salt conditions, but not when the plants get a full-strength culture medium. At low ionic strength, cultivation at 25°C gives only half as high activation as cultivation at 18°C or at high ionic strength at both temperatures. Corresponding data for activation of ATPases from oats also show that low ionic strength during growth gives the highest temperature dependence. Low temperature together with low salt conditions during growth gives the highest ATPase activity after stimulation with divalent cations. High growth temperature and full-strength medium decrease the ATPase activity. Activation energies (Ea) were calculated for the two temperature intervals 35–20°C and 20–5°C. The dominating ATPase stimulation (Ca2+ in wheat, Mg2+ in oats) is characterized by high specific activity combined with a low Ea value. The differences in ATPase activity between oats and wheat can be correlated with different cultivation requirements known from agriculture. 相似文献
35.
Embolism reversal in rice plants was studied by testing the plant's ability to refill embolized conduits while xylem pressures were substantially negative. Intact, potted plants were water-stressed to a xylem pressure of -1.88 ± 0.1 MPa and a 66.3 ± 3.8% loss of xylem conductivity (PLC) by cavitation. Stressed plants were carefully rewatered, allowing xylem pressure to rise, but not above the theoretical threshold of c. -0.15 MPa for embolism collapse. Despite xylem pressures being more negative than this threshold, the PLC fell significantly (28.5 ± 5.6%), indicating the refilling of vessels. Above c. -1.0 MPa, almost all plants regained their maximum hydraulic conductivity. Dye uptake experiments showed the same pattern of embolism refilling despite negative pressure. Refilling was prevented in plants that were light-starved for 5 d, suggesting the unknown mechanism is dependent on metabolic energy. Results are among the first showing that herbaceous plants can reverse embolism without bulk xylem pressures rising near or above atmospheric. 相似文献
36.
Genome-wide Introgression Lines and their Use in Genetic and Molecular Dissection of Complex Phenotypes in Rice (Oryza sativa L.) 总被引:15,自引:0,他引:15
Li ZK Fu BY Gao YM Xu JL Ali J Lafitte HR Jiang YZ Rey JD Vijayakumar CH Maghirang R Zheng TQ Zhu LH 《Plant molecular biology》2005,59(1):33-52
Tremendous efforts have been taken worldwide to develop genome-wide genetic stocks for rice functional genomic (FG) research
since the rice genome was completely sequenced. To facilitate FG research of complex polygenic phenotypes in rice, we report
the development of over 20 000 introgression lines (ILs) in three elite rice genetic backgrounds for a wide range of complex
traits, including resistances/tolerances to many biotic and abiotic stresses, morpho-agronomic traits, physiological traits,
etc., by selective introgression. ILs within each genetic background are phenotypically similar to their recurrent parent
but each carries one or a few traits introgressed from a known donor. Together, these ILs contain a significant portion of
loci affecting the selected complex phenotypes at which allelic diversity exists in the primary gene pool of rice. A forward
genetics strategy was proposed and demonstrated with examples on how to use these ILs for large-scale FG research. Complementary
to the genome-wide insertional mutants, these ILs opens a new way for highly efficient discovery, candidate gene identification
and cloning of important QTLs for specific phenotypes based on convergent evidence from QTL position, expression profiling,
functional and molecular diversity analyses of candidate genes, highlights the importance of genetic networks underlying complex
phenotypes in rice that may ultimately lead to more complete understanding of the genetic and molecular bases of quantitative
trait variation in rice.
Supplementary material to this paper is available in electronic form at
http://dx.doi.org/10.1007/s11103-005-8519-3 相似文献
37.
38.
QTLs for drought escape and tolerance identified in a set of random introgression lines of rice 总被引:18,自引:0,他引:18
Xu JL Lafitte HR Gao YM Fu BY Torres R Li ZK 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,111(8):1642-1650
A large set of 254 introgression lines in an elite indica genetic background were evaluated for grain yield (GY) and related
traits under the irrigated (control) and drought (stress) conditions in two consecutive years for genetic dissection of adaptive
strategies of rice to water stress. A total of 36 quantitative trait loci (QTLs) affecting heading date (HD), plant height
(PH), GY and yield components were identified and most QTLs showed pronounced differential expression either qualitatively
or quantitatively in response to drought. These QTLs could be grouped into three major types based on their behaviors under
control and stress conditions. Type I included 12 QTLs that expressed under both the stress and non-stress conditions. Type
II comprised 17 QTLs that expressed under irrigation but not under stress. Type III included seven QTLs that were apparently
induced by stress. The observation that the Lemont (japonica) alleles at all HD QTLs except QHd5 resulted in early heading under stress appeared to be responsible for the putative adaptation of Lemont to drought by escaping,
whereas the Teqing (indica) alleles at most PH/GY QTLs were consistently associated with increased yield potential and trait
stability and thus contributed to DT. Our result that most DT QTLs were non-allelic with QTLs for drought escaping suggests
that the two adaptive strategies in the parental lines are under possible negative regulation of two largely non-overlapping
genetic systems. 相似文献
39.
Lafitte D Tsvetkov PO Devred F Toci R Barras F Briand C Makarov AA Haiech J 《Biochimica et biophysica acta》2002,1600(1-2):105-110
Calmodulin is the most ubiquitous calcium binding protein. The protein is very sensitive to oxidation and this modification has pronounced effects on calmodulin function. In this work, we decided to fully oxidise calmodulin in order to study the consequences on cation binding, domain stability, and alpha helicity. Oxidation of methionines unfolds completely the apostate of the protein, which upon calcium binding recovers the major part of its secondary and tertiary structure. However, the unstructuring of the apostate results in a protein that binds calcium to any site in an independent manner, does not bind magnesium and does not possess auxiliary sites anymore. 相似文献
40.
Thomas Martinez Hélène Texier Virginie Nahoum Claude Lafitte Gianluca Cioci Laurent Heux Bernard Dumas Michael O’Donohue Elodie Gaulin Claire Dumon 《PloS one》2015,10(9)
Oomycetes are microorganisms that are distantly related to true fungi and many members of this phylum are major plant pathogens. Oomycetes express proteins that are able to interact with plant cell wall polysaccharides, such as cellulose. This interaction is thought to be mediated by carbohydrate-binding modules that are classified into CBM family 1 in the CAZy database. In this study, the two CBMs (1–1 and 1–2) that form part of the cell wall glycoprotein, CBEL, from Phytophthora parasitica have been submitted to detailed characterization, first to better quantify their interaction with cellulose and second to determine whether these CBMs can be useful for biotechnological applications, such as biomass hydrolysis. A variety of biophysical techniques were used to study the interaction of the CBMs with various substrates and the data obtained indicate that CBEL’s CBM1-1 exhibits much greater cellulose binding ability than CBM1-2. Engineering of the family 11 xylanase from Talaromyces versatilis (TvXynB), an enzyme that naturally bears a fungal family 1 CBM, has produced two variants. The first one lacks its native CBM, whereas the second contains the CBEL CBM1-1. The study of these enzymes has revealed that wild type TvXynB binds to cellulose, via its CBM1, and that the substitution of its CBM by oomycetal CBM1-1 does not affect its activity on wheat straw. However, intriguingly the addition of CBEL during the hydrolysis of wheat straw actually potentiates the action of TvXynB variant lacking a CBM1. This suggests that the potentiating effect of CBM1-1 might not require the formation of a covalent linkage to TvXynB. 相似文献